目的:本实验以紫锥菊愈伤组织上刚分化出的不定芽为材料,用不同浓度秋水仙素溶液对其进行诱导,确定最佳处理浓度和处理时间,并对诱导的多倍体与二倍体进行形态、显微、染色体及过氧化氢酶(CAT)活性的比较鉴定。结果表明:0.025%水仙素浓度处理24h的诱导效果最好,诱导率达42.85%;多倍体植株叶片肥厚、根粗壮,气孔面积极显著地大于二倍体植株,染色体数从24-28条不等,CAT平均酶活性是二倍体的2.1倍。 OBJECTIVE: In this experiment, the adventitious buds differentiated from callus of Echinacea purpurea were used as materials to induce colchicine solution with different concentrations to determine the optimal treatment concentration and treatment time. The induced polyploid and doubled The morphological, microscopic, chromosomal and catalase (CAT) activities were compared and identified. The results showed that: 0.025% of the concentration of dactylis 24h treatment induced the best, the induction rate of 42.85%; polyploid leaf hypertrophy, stout, stomata area is significantly larger than diploid plants, chromosome number from 24-28 Unequal, CAT average enzyme activity is 2.1 times diploid.