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雷公藤是一种有效的抗风湿中草药 ,临床和实验研究表明其具有多种药理学功能 .实验将 30只雄性 Sprague- Dawley大鼠完全随机分成雷公藤醋酸乙酯提取物 (TWEE)组 ,泼尼松组和对照组 .分别 ig给药 TWEE2 5mg·kg-1,每日 2次 ;泼尼松2 mg· kg-1,每日 2次和 0 .5%羧甲基纤维素钠 30d,所有大鼠于 d31断头处死 ,分离垂体和收集血液 .对垂体进行 HE和免疫细胞化学染色 ;应用放射免疫分析法检测血浆促肾上腺皮质激素 (ACTH)含量 .结果表明 TWEE能促进大鼠垂体远侧部ACTH细胞合成和分泌 ACTH.为临床综合评价雷公藤对下丘脑 -垂体 -肾上腺轴的影响提供了实验数据和理论依据 .
Tripterygium wilfordii is a potent anti-rheumatic herbal medicine, clinical and experimental studies have shown that it has a variety of pharmacological functions.Experimental 30 male Sprague-Dawley rats were randomly divided into TWEE group, Nisong group and control group were given ig TWEE2 5mg · kg-1, twice a day; prednisone 2 mg · kg-1, 2 times a day and 0.5% sodium carboxymethyl cellulose 30d, All rats were sacrificed at d31 decapitated, the pituitary gland was collected and the blood was collected.The pituitary gland was subjected to HE and immunocytochemical staining, and the content of adrenocorticotropic hormone (ACTH) in the plasma was detected by radioimmunoassay.The results showed that TWEE can promote the pituitary far Synthesis and secretion of ACTH by lateral ACTH cells provide experimental data and theoretical basis for the comprehensive evaluation of the effects of Tripterygium on the hypothalamic-pituitary-adrenal axis.