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在产毒素大肠杆菌（ＥＴＥＣ）肠毒素基因内设计合成三对引物，建立了三对引物同时ＰＣＲ检测ＥＴＥＣ的方法，一次ＰＣＲ即可扩增出６２７ｂｐ（ＬＴｈ）、２４０ｂｐ（ＳＴＩａ）和１６９ｂｐ（ＳＴＩｂ）三种肠毒素基因片段，可同时分型检测出ＬＴｈ、ＳＴＩａ、ＳＴＩｂ、ＬＴｈ－ＳＴＩａ、ＬＴｈ－ＳＴＩｂ五种基因型的ＥＴＥＣ，与非ＥＴＥＣ对照菌无交叉反应，最小检出量为１０ｃｆｕ，显示了很高的特异性和敏感性。将建立的方法用于山东省六县市６２３例大肠杆菌致泻标本的检测，阳性率为４０．３％，并能鉴别ＥＴＥＣ的基因型，为ＥＴＥＣ腹泻的分子流行病学研究提供了有效的检测手段
Three pairs of primers were designed and synthesized in the enterotoxin gene of toxin-producing Escherichia coli (ETEC). Three pairs of primers were designed to detect ETEC simultaneously. One PCR amplified 627bp (LTh), 240bp (STIa) and 169bp ) Three kinds of enterotoxin gene fragments can be detected at the same time LTh, STIa, STIb, LTh-STIa, LTh-STIb ETEC five genotypes and non-ETEC control bacteria no cross-reaction, the minimum detectable amount of 10cfu, Shows high specificity and sensitivity. The established method was applied to the detection of 623 Escherichia coli diarrhea specimens in six counties of Shandong Province with the positive rate of 40.3% and the genotypes of ETEC were identified, which provided an effective molecular epidemiological study on ETEC diarrhea testing method