胰岛素样生长因子-Ⅰ(IGF-Ⅰ)具有重要的生物学作用,在许多疾病状态下。血清和组织中的IGF-Ⅰ含量都有变化。因此,IGF-Ⅰ含量测定具有临床应用的潜力。一、材料与方法 1.重组IGF-Ⅰ购自美国Promage公司,兔抗IGF-Ⅰ血清由美国糖尿病消化病肾病研究所惠赠,Na~(125)I购自中国原子能科学研究院。 2.~(125)I-IGF-Ⅰ的制备采用Iodogen法,在事先涂有Iodogen(20μg)的试管中同时加入2μg IGF-Ⅰ和37MBq的Na~(125)I,反应 Insulin-like growth factor-I (IGF-I) has an important biological role in many disease states. IGF-I levels vary in serum and tissue. Therefore, the determination of IGF-Ⅰ has the potential of clinical application. Materials and Methods 1. Recombinant IGF-Ⅰ was purchased from Promage Inc. of the United States. Rabbit anti-IGF-Ⅰ serum was favored by the American Society of Diabetic Nephropathy, Na ~ (125) I was purchased from China Institute of Atomic Energy. 2. Preparation of 125 I-IGF-I Using the Iodogen method, 2 μg of IGF-I and 37 MBq of Na ~ (125) I were simultaneously added into a test tube coated with Iodogen (20 μg)