利用PCR基因扩增法,以SARS冠状病毒全基因质粒为模板,获得N蛋白相应抗原基因,构建了表达载体pBV220/SARS-N,并在E.coli中获得高效表达。用纯化后的N蛋白抗原包被测定板,通过间接ELISA法对阴阳性血清进行活性测定,结果表明,在46份阳性血中有41份被测出,检出率为89.13%。本研究克隆并表达了SARS冠状病毒N蛋白,为进一步研制SARS病人抗体检测试剂和SARS疫苗奠定了基础。 The full length of SARS coronavirus plasmid was used as a template to obtain the corresponding antigen of N protein by PCR amplification. The expression vector pBV220 / SARS-N was constructed and highly expressed in E.coli. The plate was coated with the purified N protein antigen, and the activity of the negative and positive serum was determined by an indirect ELISA. The results showed that 41 of the 46 positive blood samples were detected with a detection rate of 89.13%. This study cloned and expressed the SARS-Covirus N protein, which laid the foundation for the further development of SARS antibody and SARS vaccine.