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目的:探讨耐药相关蛋白P-糖蛋白(P-gp)和谷胱甘肽S转移酶(GST-π)在胆道恶性肿瘤组织中的表达及其与临床病理参数的相关性。方法:采用免疫组化SP法检测60例胆道恶性肿瘤组织中P-gp和GST-π的表达。结果:60例胆道恶性肿瘤组织P-gp阳性表达率为88.3%,GST-π阳性表达率为93.3%。肿瘤高、中分化组织P-gp和GST-π阳性表达率分别为79.3%和86.2%,均低于低分化组织的96.8%和100.0%,P值均<0.05;局部浸润组织P-gp和GST-π阳性表达率分别为96.4%和100.0%,均高于无局部浸润组织的81.3%和87.5%,P值均<0.05;淋巴转移组织P-gp和GST-π阳性表达率分别为97.0%和100.0%,均高于无淋巴转移的77.8%和85.2%,P值均<0.05。结论:P-gp和GST-π在胆道恶性肿瘤组织中呈高表达,联合检测P-gp和GST-π对指导临床化疗药物、制定合理的个体化疗方案和判断预后有着重要的意义。
Objective: To investigate the expression of P-glycoprotein (P-gp) and glutathione S-transferase (GST-π) in cholangiocarcinoma and its correlation with clinicopathological parameters. Methods: The expression of P-gp and GST-π in 60 cases of cholangiocarcinoma was detected by immunohistochemical SP method. Results: The positive expression rate of P-gp in 60 cases of cholangiocarcinoma was 88.3% and the positive rate of GST-π was 93.3%. The positive rates of P-gp and GST-π in high and moderately differentiated tumor tissues were 79.3% and 86.2%, respectively, which were lower than 96.8% and 100.0% in poorly differentiated tissues, respectively, with P values <0.05; P-gp and The positive expression rate of GST-π was 96.4% and 100.0%, respectively, which were higher than 81.3% and 87.5% of those without local infiltration. The positive rates of P-gp and GST-π in lymph node metastasis were 97.0 % And 100.0% respectively, which were higher than 77.8% and 85.2% respectively without lymph node metastasis (P <0.05). Conclusions: P-gp and GST-π are highly expressed in cholangiocarcinoma tissues. Combined detection of P-gp and GST-π is of great significance in guiding clinical chemotherapy drugs, formulating rational individual chemotherapy regimen and prognosing prognosis.