在酸性Tris-盐酸缓冲介质中,孔雀石绿与呋塞米反应生成了具有3个峰值相近的负吸收峰的绿色离子缔合物,其波长分别位于652,554和424 nm,表观摩尔吸光系数分别为2.21×10~4,2.28×10~4和1.96×10~4L/(mol·cm)。当用三波长法测定时,表观摩尔吸光系数(ε)可达6.45×10~4L/(mol·cm)。呋塞米的质量浓度在0~5.1 mg/L内遵从比尔定律。方法适用于人体血液、尿液及药物中呋塞米的测定。 In acidic Tris-HCl buffer medium, malachite green reacted with furosemide to generate green ion-association complexes with three negative absorption peaks with similar peak wavelengths at 652, 554 and 424 nm, respectively. The apparent molar absorptivities 2.21 × 10 ~ 4,2.28 × 10 ~ 4 and 1.96 × 10 ~ 4L / (mol · cm). The apparent molar absorptivity (ε) can reach 6.45 × 10 ~ 4L / (mol · cm) when measured by three wavelength method. Beer’s law is obeyed at a concentration of 0 to 5.1 mg / L for furosemide. The method is suitable for the determination of furosemide in human blood, urine and medicine.