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采用药液浸渍法、沙柱法以及与荧光染料Cy3共孵育的方法,以丙溴磷、克百威和阿维菌素为对照药剂,测定了溴氰菊酯对马铃薯腐烂茎线虫Ditylenchus destructor的毒力以及对其运动扩散和摄食的影响。结果表明:溴氰菊酯对马铃薯腐烂茎线虫具有一定的杀灭活性,其LC_(50)值为459.8 mg/L,活性低于丙溴磷(159.9 mg/L)、克百威(331.9 mg/L)和阿维菌素(257.3 mg/L);溴氰菊酯对马铃薯腐烂茎线虫运动扩散的抑制作用IC_(50)值为3.1 mg/L,其活性低于阿维菌素(0.8 mg/L),但高于丙溴磷(8.3 mg/L)和克百威(16.1 mg/L)。当丙溴磷和克百威质量浓度分别低至0.6和40 mg/L时,可刺激90%以上的线虫摄食;当丙溴磷和克百威质量浓度最低分别为360和300 mg/L时,可抑制全部线虫的摄食;溴氰菊酯与阿维菌素则对线虫的摄食无刺激作用;用10 mg/L的丙溴磷处理线虫2 h后再分别用阿维菌素和溴氰菊酯处理,发现阿维菌素和溴氰菊酯质量浓度最低分别为20和200 mg/L时可抑制全部马铃薯腐烂茎线虫的摄食。研究表明,溴氰菊酯对马铃薯腐烂茎线虫具有较高的活性,其在线虫防治领域的开发应用潜力较好。
The liquid immersion method, the sand column method and the method of incubation with the fluorescent dye Cy3, with profenofos, carbofuran and avermectin as the control agent, measured deltamethrin of Ditylenchus destructor Toxicity and its effects on movement and food intake. The results showed that deltamethrin had some antibacterial activity against D. dipsaci, its LC 50 value was 459.8 mg / L, its activity was lower than that of profenofos (159.9 mg / L) and carbofuran (331.9 mg / L) and abamectin (257.3 mg / L). The inhibitory effect of deltamethrin on the movement of D. sojae was 3.1 mg / L, which was lower than abamectin (0.8 mg / L), but higher than profenofos (8.3 mg / L) and carbofuran (16.1 mg / L). When the concentrations of profenofos and carbofuran were as low as 0.6 and 40 mg / L, respectively, they could ingest more than 90% of the nematodes. When the concentrations of profenofos and carbofuran were 360 and 300 mg / L respectively , Which inhibited the feeding of all nematodes. While deltamethrin and abamectin did not stimulate nematode feeding, nematodes were treated with 10 mg / L of brevifosin for 2 h and then treated with abamectin and cyanogen bromide Inulin treatment, it was found that the lowest concentrations of abamectin and deltamethrin of 20 and 200 mg / L, respectively, inhibited all P. destructor to seize. Studies have shown that deltamethrin has high activity against D. destructor and its potential for development and application in the field of nematode control is good.