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文中将会描述一种来源于蜘蛛丝MiSp1蛋白序列的九肽经过自组装过程所形成的树枝状分形结构材料。通过原子力显微镜(AFM)和透射电子纤维镜(TEM)分别观测到了该分形结构,通过KCl的添加与否和调节溶液的pH值可以控制该分形结构的形成。圆二色谱仪(CD)的检测结果表明,在KCl存在的条件下,九肽组装体的二级结构为无规则卷曲结构,并且在20℃~80℃的范围内比较稳定。该发现不仅提供了一种新型材料,也使人们对于蜘蛛丝的形成过程有了更进一步的理解,这对于微观结构材料的构建有重要的参考价值,其应用方面的研究正在进行。
In this paper, we will describe a dendritic fractal structure of a nonapeptide derived from the spider silk MiSp1 protein sequence after self-assembly. The fractal structure was observed by atomic force microscopy (AFM) and transmission electron microscopy (TEM) respectively. The formation of the fractal structure can be controlled by the addition of KCl and the pH of the solution. Circular dichroism spectroscopy (CD) test results showed that in the presence of KCl, the secondary structure of the nonapeptide assembly has a random coil structure and is relatively stable in the range of 20 ° C to 80 ° C. The discovery not only provides a new type of material, but also gives people a better understanding of the formation process of spider silk, which has important reference value for the construction of microstructural materials and its application research is underway.