论文部分内容阅读
为探讨金属硫蛋白（MT）对豚鼠乳头肌缺血再灌注损伤所致心律失常的影响，利用标准玻璃微电极技术，采用缺氧及复氧豚鼠乳头肌模型，模拟体内缺血再灌注损伤，观察不同浓度MT对豚鼠乳头肌电生理特性的影响。结果显示低浓度的MT（0．002mmol／L）对正常及缺氧和复氧豚鼠乳头肌的动作电位（AP）有关参数及自律性均无影响；中等浓度的MT（0．02mmol／L）仅使正常乳头肌的AP复极达50％时程（APD50）缩短24％（P＜0．05），但使缺氧乳头肌的AP复极达20％时程（APD20）、APD50和AP复极达90％时程（APD90）分别缩短68％、56％和43％（P均＜0．01），并使静息电位（RP）、AP幅值（APA）和0相最大上升速率（Vmax）分别增加30％、30％和45％（P均＜0．01）；高浓度的MT（0．1mmol／L）使正常豚鼠乳头肌的APD20、APD50和APD90分别缩短57％、54％和50％（P均＜0．01），并且RP、APA及Vmax分别下降22％（P＜0．05）、28％（P＜0．01）和29％（P＜0．05），而使缺氧豚鼠乳头肌的APD20、APD50和APD90分别延长92％、78％和50％（P均＜0．01），对RP、APA及Vmax无明显影响。在复氧期间，0．02mmol／L的MT可使自律性的发生率从77．8％降至55．6％（P＜0．05）；而0．1mmol／L的MT则使自律性的发生率从77.8％降至22．2％（P
To investigate the effect of metallothionein (MT) on arrhythmia induced by ischemia-reperfusion injury in guinea pig papillary muscles, hypoxia-reoxygenation guinea pig papillary muscle model was used to simulate the ischemia-reperfusion injury in vivo by using standard glass microelectrode technique. The effects of different concentrations of MT on the electrophysiological characteristics of guinea pig papillary muscles were observed. The results showed that low concentrations of MT (0.002mmol / L) had no effect on AP parameters and self-discipline of normal and hypoglycemic and reperfused guinea pig papillary muscles. Middle concentrations of MT (0.02mmol / L) (APD50) was reduced by 24% (P <0.05), but the apodemus in hypodermic papillary muscles reached 20% APD20, APD50 and AP The APD 90%, APD 90 were reduced by 68%, 56% and 43% respectively (P <0.01), and the resting potential (RP), AP amplitude (Pmax <0.01). High concentrations of MT (0.1 mmol / L) shortened the APD20, APD50 and APD90 of normal guinea pig papillary muscles by 57%, 54% % And 50% respectively (all P <0.01), and RP, APA and Vmax decreased by 22% (P <0.05), 28% , While prolonged APD20, APD50 and APD90 in papillary muscle of hypoxic guinea pigs by 92%, 78% and 50%, respectively (all P <0.01), but had no effect on RP, APA and Vmax. During reoxygenation, 0.02 mmol / L of MT reduced the rate of self-regulation from 77.8% to 55.6% (P <0.05), whereas 0.1 mmol / L of MT reduced self-regulation The incidence decreased from 77.8% to 22.2% (P