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目的观察三氧化二砷(Arsenic trioxide,As_2O_3)对人喉鳞癌细胞株Hep-2细胞的作用,为晚期喉癌的综合治疗提供实验依据。方法以不同浓度的As_2O_3作用于Hep-2,利用细胞生长曲线、MTF法、瑞氏-吉姆萨(Wrighs-Gimesa)染色,DNA凝胶电泳技术,TUNEL,流式细胞术,观察其细胞增殖抑制、凋亡及细胞周期作用。结果 As_2O_3对Hep-2具有增殖抑制作用,显示时间和剂量效应。在一定剂量和时间范围内,主要引起细胞凋亡,并具有药物浓度依赖性,细胞大部分阻滞在G_2+M期。结论 As_2O_3对Hep-2具有增殖抑制及诱导调亡的双重作用,为喉癌治疗提供了一个具有可行性的设想和依据。
Objective To observe the effect of Arsenic trioxide (As_2O_3) on human laryngeal squamous cell carcinoma cell line Hep-2 and provide experimental evidence for the comprehensive treatment of advanced laryngeal carcinoma. Methods Hep-2 cells were treated with different concentrations of As 2 O 3. Cell growth inhibition, MTT assay, Wrighs-Gimesa staining, DNA gel electrophoresis, TUNEL and flow cytometry were used to observe the cell proliferation inhibition , Apoptosis and cell cycle. Results As_2O_3 inhibited the proliferation of Hep-2 cells and showed time and dose effects. In a certain dose and time range, the main cause apoptosis, and drug concentration-dependent, the majority of cells in the G 2 M phase. Conclusion As_2O_3 has the dual role of inhibiting proliferation and inducing apoptosis of Hep-2, which provides a feasible idea and basis for the treatment of laryngeal cancer.