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目的:以黄芩归经为指导,探索清开灵注射液(QKL)的效应物质。方法:取大鼠,给药后于不同时间点处死,取脑、肺、肝。另取大鼠,分为正常组和缺血再灌注组,各组大鼠按采样点随机分为5组,每组5只。正常组大鼠经尾静脉给予800 mg.kg-1QKL。脑缺血再灌注组采用改良的腔内线拴法通过阻闭大脑中动脉120 min后再灌注120 min复制局灶性脑缺血-再灌注病理模型,并于恢复再灌注120 min时给予800 mg.kg-1QKL。给药后于不同时间点处死,取脑、肺、肝。HPLC-MS法测定黄芩苷浓度。结果:正常大鼠注射清开灵后,大量黄芩苷很快分布至肺、肝中,脑中分布较少。脑缺血-再灌注大鼠脑中黄芩苷量较正常组明显升高。结论:黄芩苷为清开灵治疗肝、肺、脑缺血疾病的效应物质之一;以中药归经为指导,进行复方效应物质探索具有可行性。
OBJECTIVE: To explore the effect of Qingkailing Injection (QKL) with the guidance of Scutellaria baicalensis. Methods: Rats were sacrificed at different time points after administration, and brain, lung and liver were taken. Another rat was divided into normal group and ischemia-reperfusion group. The rats in each group were randomly divided into 5 groups according to sampling points, with 5 rats in each group. Normal rats were given 800 mg.kg-1 QKL through tail vein. The model of focal cerebral ischemia-reperfusion was established by occlusion of the middle cerebral artery occlusion for 120 min after cerebral ischemia and reperfusion with 120 min reperfusion followed by 120 min reperfusion. .kg-1QKL. After administration at different time points sacrifice, take the brain, lung, liver. Determination of baicalin concentration by HPLC-MS method. Results: After the normal rats were injected Qingkailing, a large number of baicalin quickly distributed to the lungs, liver, brain distribution is less. The amount of baicalin in the brain of cerebral ischemia-reperfusion rats was significantly higher than that of the normal group. Conclusion: Baicalin is one of the effective substances for Qingkailing in the treatment of liver, lung and cerebral ischemia. Guided by traditional Chinese medicine, it is feasible to explore the compound effect substances.