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丛枝菌根真菌(AMF)能够显著提高植物对重金属的抗性,菌丝内聚磷酸(Polyphosphate,PolyP)可能参与了这种抗性的形成。试验以Glomus intraradices孢子为试材,对灭菌条件进行了优化,并进一步研究了不同P和Cd2+水平对孢子萌发、菌丝生长、分支和外生菌丝中聚磷酸含量的影响。结果表明,孢子在1%氯胺T+0.02%链霉素+0.01%庆大霉素+1/100(V/V)吐温-20中灭菌5min的效果最好。孢子萌发率、菌丝分支和菌丝长度随着Cd2+浓度的增加不断降低;当Cd2+浓度达到0.1mmol/L时,孢子萌发率降低为0%,表明Glomus intraradices的孢子萌发对Cd2+的耐受极限为0.1mmol/L;1mmol/L的P促进菌丝分支增加,却降低了萌发率,但对菌丝生长没有影响;在培养23d以后,三者基本不再变化。外生菌丝内的聚磷酸含量随着P的升高而增加;在Cd2+胁迫作用下,聚磷酸的含量降低而菌丝密度随着聚磷酸的升高而升高,表明聚磷酸在减弱重金属毒性方面起了重要作用。
Arbuscular mycorrhizal fungi (AMF) significantly increased plant resistance to heavy metals, and polyphosphate (PolyP), a mycelium, may be involved in the formation of this resistance. In the experiment, the spores of Glomus intraradices were used as experimental material to optimize the sterilization conditions and further study the effects of different P and Cd2 + levels on the spore germination, mycelial growth, branching and exogenous mycelium polyphosphate content. The results showed that spores were best for sterilization in 1% chloramine T + 0.02% streptomycin + 0.01% gentamycin +1/100 (V / V) Tween-20 for 5 min. The spore germination rate, mycelium branching and mycelial length decreased with the increase of Cd2 + concentration. When the concentration of Cd2 + reached 0.1mmol / L, the spore germination rate was reduced to 0%, indicating that the tolerance of spores of Glomus intraradices to Cd2 + 0.1 mmol / L; 1 mmol / L P promoted the growth of mycelial branches, but decreased the germination rate, but had no effect on mycelial growth. After cultured for 23 days, the three basically did not change. The content of polyphosphoric acid in exogenous mycelium increased with the increase of P. Under Cd2 + stress, the content of polyphosphoric acid decreased while the mycelial density increased with the increase of polyphosphoric acid Toxicity has played an important role.