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本实验选用具有生育力成年雄性猕猴７只，在直视下行双侧ＨＦＭＣ输精管内注射，每侧剂量分别为３０ｍｇ１只，６０ｍｇ和１００ｍｇ各３只；于注射后２．５年和３．５年分别处死动物，取睾丸组织进行光镜和电镜观察．结果发现：猕猴注射ＨＦＭＣ２．５年后，睾丸光镜大部分曲细精管生精上皮结构完整，排列整齐。仅见局部少数管腔生精上皮层数减少，上皮细胞轻度水样变性等病理改变。电镜下曲细精管内除支持细胞内脂褐素增多，轻度基底膜增厚和精母细胞内质网扩张外，各级生精细胞，支持细胞及细胞间连接复合体等超微结构未见明显异常。注射ＨＦＭＣ３．５年后猕猴的光镜、电镜结果与注射后２．５年结果相似，但局部改变较２．５年组轻。上述结果表明：猕猴输精管内注射一定剂量ＨＦＭＣ节育不会引起睾丸组织的严重病理改变。但是，由于注射ＨＦＭＣ后，ＨＦＭＣ释放Ｈ＋及其对输精管的暂时阻塞，改变了精子生存的内环境，使睾丸出现局部轻度病理改变，随着ＨＦＭＣ逐渐溶解排出，睾丸功能相继恢复正常，配对产仔。为ＨＦＭＣ应用提供了安全性依据。
In this experiment, 7 male adult macaques with fertility were selected and injected into bilateral vas deferens under direct vision with doses of 30mg1, 60mg and 100mg on each side respectively. After 2.5 and 3.5 years of injection Animals were sacrificed, testicular tissue were taken for light and electron microscopy. The results showed that most of the seminiferous tubules of seminiferous epithelium were intact and arranged neatly after 2.5 years of HFMC injection. Only a small number of local luminal spermatogenic epithelial layer to reduce the number of mild epithelial cell degeneration and other pathological changes. Under electron microscope, in addition to the support of intracellular increase of lipofuscin, thickening of basement membrane and endoplasmic reticulum expansion of spermatocytes, the ultrastructure of seminiferous tubules, supporting cells and intercellular junctional complexes See obvious abnormalities. The results of electron microscopy and electron microscopy 2.5 years after injection of HFMC 3.5 years after HFMC injection were similar, but the local changes were lighter than those of 2.5 years. The above results show that intramuscular injection of a dose of HFMC in vas deferens does not cause serious pathological changes in testicular tissue. However, HFMC releases H + and temporarily obstructs the vas deferens after HFMC injection, alters the internal environment of the sperm and makes the testes locally mild pathological changes. As the HFMC gradually dissolves and excretes, the testis functions return to normal one after another, kid. Provides a safety basis for HFMC applications.