论文部分内容阅读
目的 :研究细胞容积调节机制 ,探讨人类小肠上皮细胞调节性容积减小 (RVD)过程中离子通道的作用及其种类。方法 :膜片钳全细胞记录和单通道记录法记录培养的人类小肠上皮细胞 RVD过程中电流的变化 ,细胞容积测定法观察 RVD过程中特异性钙激活性钾通道阻断剂 (clotrimazole)的作用。结果 :全细胞记录法证实细胞 RVD过程中 K+通道和 Cl-通道电流同时被激活 ,该 K+通道电流具有明显的钙依赖性并可被 clotrim azole阻断。单通道记录法进一步证实 RVD过程中激活的 K+通道为 Interm ediate- conductance钙激活性钾通道。结论 :人类小肠上皮细胞在低渗溶液作用下具有 RVD过程 ,钙激活性钾通道在 RVD过程中具有十分重要的作用。
OBJECTIVE: To study the mechanism of cell volume regulation and to explore the roles and types of ion channels in the regulation of the volume reduction (RVD) of human intestinal epithelial cells. Methods: Whole-cell patch clamp recording and single-channel recording method were used to record the changes of current in cultured human intestinal epithelial cells during RVD. The effects of specific calcium-activated potassium channel blockers (Clotrimazole) during RVD were observed by cell volume assay . Results: K + channel and Cl-channel currents were activated by whole-cell recording at the same time in RVD. The K + channel currents were obviously calcium-dependent and blocked by clotrim azole. The single-channel recording method further confirmed that the K + channel activated in the RVD process is an Intermittent conductance calcium-activated potassium channel. Conclusion: Human intestinal epithelial cells have RVD under hypotonic solution, and calcium-activated potassium channel plays an important role in RVD.