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应用免疫酶标细胞化学技术在雌激素受体蛋白特异的5种大鼠单克隆抗体中分别对人乳腺肿瘤、人子宫、兔子宫等的冰冻切片进行雌激素受体蛋白(亲雌素)定位,可观察到核特异性的染色还可观察到染色有对单克隆抗体的存在与否及浓度,的依赖性,以及这种染色可被高度纯化的亲雌素竞争性抑制。非靶组织如结肠上皮细胞的染色总是阴性的,MCF-7细胞如同大多数人乳腺肿瘤一样对亲雌素的特异染色是不均一的,仅局限于核。染色的不均一性可能反映这些样品源自多克隆,或许也与细胞群的生理状态(如细胞周期)有关.迄今为止,在任何被测定的组织或肿瘤细胞中包括由于绝
Immunocytochemistry was used to locate the estrogen receptor protein (estrogen receptor) in frozen sections of human breast tumors, human uterus, rabbit uterus and so on in 5 rat monoclonal antibodies specific to estrogen receptor protein , Nuclear-specific staining can be observed as well as the presence and concentration of the monoclonal antibody depending on the staining, and this staining can be competitively inhibited by a highly purified pro-estrogen. Staining of non-target tissues, such as colonic epithelial cells, is always negative and MCF-7 cells are heterogeneous for specific staining for progestins just like most human breast tumors, confined only to the nucleus. Staining heterogeneity may reflect that these samples originated from polyclonal and may also be related to the physiological status of the cell population (eg, cell cycle) To date, the inclusion of any of the identified tissues or tumor cells