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在水温(25±1)℃条件下,暂养1周后将青虾在30 mg/L的五氯酚钠溶液中浸泡1 h,以转移入清水的时间为零点,按照1/6、0.5、1、2、4、8、12、24、36、48、72、96、120、144、168、192、288、384、480、720 h采样点采样,采用气相色谱法测定青虾肌肉组织和肝胰腺中五氯酚钠含量,研究五氯酚钠在青虾体内的药代动力学。结果发现五氯酚钠药物在青虾体内的药-时曲线可以用三室开放模型来描述。当青虾浸泡在五氯酚钠溶液1h后,青虾体内的五氯酚钠浓度达到最大,其中,肝胰腺中的五氯酚钠浓度高于肌肉。主要药物动力学参数为:肌肉与肝胰腺的表观分布容积(Vd)分别为0.117 0、9.588 0(μg/kg)/(μg/kg),分布半衰期(T1/2α)分别为4.92、29.13 h,消除半衰期(T1/2β)分别为31.69、58.21 h,药-时曲线下面积(AUC)分别为9.1264×103、5.071 5×104μg/(kg.h),总体消除率CL(s)分别为0.035、0.197[μg/(kg.h)]/(μg/kg)。结果显示,五氯酚钠在青虾体内分布吸收较迅速,但消除相对比较缓慢,具有较长的消除半衰期。
Under the condition of water temperature (25 ± 1) ℃, the freshwater shrimp was immersed for 1 h in 30 mg / L sodium pentachlorophenate solution after holding for 1 week, and the time of transferring into fresh water was zero. According to 1 / 6,0.5 , 1,2,4,8,12,24,36,48,72,96,120,144,168,192,288,384,480,720 h sampling point sampling, gas chromatography Determination of mussel tissue And hepatopancreas sodium pentachlorophenate content of sodium pentachlorophenate shrimp in vivo pharmacokinetics. The results showed that the drug-time curve of sodium pentachlorophenol in shrimp can be described by a three-compartment open model. When freshwater shrimp immersed in sodium pentachlorophenate solution for 1h, the concentration of sodium pentachlorophenate reached the maximum in the shrimp, among them, the concentration of sodium pentachlorophenate in hepatopancreas was higher than that in muscle. The main pharmacokinetic parameters of muscle and hepatopancreas were 0.117 0 and 9.588 0 (μg / kg) / (μg / kg) respectively, and the distribution half-life (T1 / 2α) were 4.92 and 29.13 h, and the elimination half-life (T1 / 2β) were 31.69 and 58.21 h respectively. The area under the drug-time curve (AUC) was 9.1264 × 103 and 5.071 × 104μg / (kg.h) Was 0.035 and 0.197 [μg / (kg.h)] / (μg / kg). The results showed that sodium pentachlorophenate absorbed more rapidly in the freshwater shrimp, but its elimination was relatively slow and had a longer elimination half-life.