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目的:探讨宫颈癌患者淋巴结HPV16/18感染及术前血清鳞状细胞癌抗原(SCCA)水平与临床病理参数的相关性,以及宫颈分泌物及淋巴结中HPV16/18感染和术前血清SCCA水平三者之间的关系。方法:采用实时荧光定量PCR法检测35例宫颈癌患者的宫颈分泌物及淋巴结HPV16/18阳性率,同时用ELISA法检测术前血清SCCA水平。结果:宫颈癌患者宫颈分泌物及淋巴结HPV16/18阳性率分别为80%(28/35)和20%(7/35)。7例组织学阳性淋巴结及28例阴性淋巴结HPV16/18阳性率分别为85.71%(6/7)和3.57%(1/28)。SCCA阳性率为37.14%(13/35)。淋巴结HPV16/18阳性感染与术前SCCA水平相关(r=0.650,P<0.01),两者均与淋巴结转移、间质浸润深度及脉管累及有关(P<0.05),而和宫颈分泌物HPV16/18阳性感染无关(P>0.05)。结论:HPV16/18在组织学阳性淋巴结中感染率高,与宫颈癌患者淋巴结转移密切相关。而阴性淋巴结中的HPV16/18检出提示微转移。术前SCCA水平与淋巴结转移相关。
Objective: To investigate the correlation between HPV16 / 18 infection and the level of SCCA in cervical lymph nodes and clinicopathological parameters, and the relationship between HPV16 / 18 infection in cervical secretions and lymph nodes and the level of preoperative serum SCCA The relationship between people. Methods: The positive rates of HPV16 / 18 in cervical secretions and lymph nodes were detected by real-time fluorescence quantitative PCR in 35 cases of cervical cancer. Serum SCCA levels were measured by ELISA. Results: The positive rates of HPV16 / 18 in cervical secretions and lymph nodes of cervical cancer patients were 80% (28/35) and 20% (7/35), respectively. The positive rates of HPV16 / 18 in seven cases of histologically positive lymph nodes and 28 cases of negative lymph nodes were 85.71% (6/7) and 3.57% (1/28), respectively. The positive rate of SCCA was 37.14% (13/35). The HPV16 / 18 positive infection in lymph node was correlated with preoperative SCCA level (r = 0.650, P <0.01), both of which were related to lymph node metastasis, depth of interstitial infiltration and vascular involvement (P <0.05) / 18 positive infection (P> 0.05). Conclusion: HPV16 / 18 has a high infection rate in histologically positive lymph nodes and is closely related to lymph node metastasis in cervical cancer patients. While HPV16 / 18 detection in negative lymph nodes suggests micrometastasis. Preoperative SCCA levels are associated with lymph node metastases.