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荧光寿命法成像技术(FLIM)是一种非常有效、功能强大且能用来分析复杂生物组织和细胞分子的成像技术。传统的荧光寿命成像的数据分析,按某些具有不同寿命、离散的单参量指数模型来描述荧光衰减过程。在生物组织这样既复杂又不均匀的样品中,虽然多参量指数模型能提供比单参量指数模型对实验数据更好的拟合效果,但是离散多参量的假定往往是随意的。提出了拟威布尔分布密度函数可能是生物荧光分子团衰减动力过程的真实再现,并且通过计算证明,对于某些生化感兴趣的荧光分子团的多槽基面效价测定样品的数据,相对于单参量指数与多参量指数衰减函数有更好的一致性。同时讨论了将该荧光衰减模型应用于荧光寿命成像的前景。
Fluorescence lifetime imaging (FLIM) is a very effective and powerful imaging technique that can be used to analyze complex biological tissues and cell molecules. Traditional fluorescence lifetime imaging data analysis, according to some different life expectancy, discrete single-parameter exponential model to describe the fluorescence decay process. In complex and inhomogeneous samples such as biological tissue, although the multi-parameter exponential model can provide a better fitting effect than the single-parameter exponential model on the experimental data, the assumption of discrete multi-parameter is often arbitrary. It is proposed that the distribution function of quasi-Weibull may be a true representation of the dynamic process of the decay of biological fluorophores and that the data of the multichannel basal surface potency assay sample of some fluorescent molecular groups of interest for some biochemicals is proved by calculation. Single parameter index and multi-parameter exponential decay function have better consistency. The prospect of applying this fluorescence decay model to fluorescence lifetime imaging is also discussed.