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目的:研究普洱熟茶(发酵)和普洱绿茶(未发酵)这 2 种常见普洱茶的抗 HIV 作用。方法:采用 MTT 比色法检测普洱茶提取物对 C8166, MT4 和 PBMC 细胞的毒性作用; 细胞病变法检测化合物对 HIV-1 急性感染的抑制活性。采用细胞病变法和 HIV-1 p24 抗原 ELISA 方法检测普洱茶提取物的抗 HIV 活性作用; 结果:所有普洱茶提取物都具有较低的细胞毒性 CC50为 120.63 524.95 μg·mL 1。所有普洱茶提取物均能抑制 HIV 诱导细胞形成合胞体其 EC50为 11.13 67.49 μg·mL 1。普洱茶水提物的抑制作用比醇提物的抑制作用要好, 并且普洱熟茶的抑制作用好于普洱生茶, 尤其是 YYP-31, 其 SI 值为 42.40。YYP-31 对HIV-RF和 HIV-2CBL-20也有很好的抑制作用, 其 EC50分别为 30.82 和 39.79 μg·mL 1。YYP-31 还能很好地抑制 HIV-1IIIB急性感染C8166 细胞和 HIV-1KM018感染PBMC细胞p24抗原的产生, 其EC50分别为14.95和 74.63 μg·mL 1。YYP-31能阻止正常细胞C8166与 HIV-1 慢性感染细胞 H9/HIV-1IIIB之间的融合, EC50为 234.27 μg·mL 1。提取物 YYP-31 与 AZT 联合用药具有显著的协同抗HIV 作用,但是对 HIV 逆转录没有抑制作用。结论:普洱茶提取物具有很好的抗 HIV 的活性作用, 可以作为一种 HIV 辅助治疗的产品或保健品用于 HIV 患者的治疗过程中。
Objective: To study the anti-HIV effect of Puer tea (fermented) and Pu’er green tea (unfermented), two kinds of common Pu’er tea. Methods: MTT colorimetric method was used to detect the cytotoxicity of Pu-erh tea extract on C8166, MT4 and PBMC cells. The cytopathic effect was used to test the inhibitory activity of the compound against HIV-1 acute infection. The anti-HIV activity of Pu-erh tea extract was detected by cytopathic assay and HIV-1 p24 antigen ELISA. Results: All Pu-erh tea extracts had lower cytotoxicity CC50 of 120.63 524.95 μg · mL -1. All Pu-erh tea extracts inhibited the HIV-induced cell formation of syncytia with an EC50 of 11.13 67.49 μg · mL -1. The inhibitory effect of Pu’er tea water extract was better than that of alcohol extract, and Pu’er tea had better inhibitory effect than Pu’er tea, especially YYP-31 with SI value of 42.40. YYP-31 also has good inhibitory effect on HIV-RF and HIV-2CBL-20 with EC50 of 30.82 and 39.79 μg · mL -1, respectively. YYP-31 also inhibits the production of p24 antigen in C8166 cells infected with HIV-1IIIB and PBMCs infected with HIV-1KM018 with EC50 of 14.95 and 74.63 μg · mL -1, respectively. YYP-31 blocked the fusion between normal cell C8166 and HIV-1 chronic infected cells H9 / HIV-1IIIB with an EC50 of 234.27 μg · mL -1. The combination of extract YYP-31 and AZT has significant synergistic anti-HIV effect, but has no inhibitory effect on HIV reverse transcription. Conclusion: Pu-erh tea extract has a good anti-HIV activity and can be used as a HIV-assisted product or health product in the treatment of HIV patients.