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目的：了解大鼠胃肠道胃泌素（ｇａｓｔｒｉｎ，Ｇ）细胞和促肾上腺皮质激素免疫反应性（ａｄｒｅｎｏｃｏｒｔｉｃｏｔｒｏｐｈｉｃｈｏｒｍｏｎｅｉｍｍｕｎｏｒｅａｃｔｉｖｉｔｙ，ＡＣＴＨＴＲ）细胞年龄变化特点及胃肠道与脑垂体ＡＣＴＨＩＲ细胞年龄变化的关系。方法：应用ＰＡＰ免疫组织化学方法和胶金免疫电镜技术。结果：１８个月大鼠胃窦Ｇ细胞及十二指肠和脑垂体ＡＣＴＨＩＲ细胞数量均分别显著低于其它组（Ｐ＜００５；Ｐ＜００１）。２５个月大鼠十二指肠ＡＣＴＨＩＲ和胃窦Ｇ细胞数量为最高值，脑垂体ＡＣＴＨＩＲ细胞数量最高值为３５天大鼠。此外，在胶金免疫电镜下，可见十二指肠杯状细胞内含有胶金颗粒。结论：大鼠十二指肠和脑垂体ＡＣＴＨＩＲ细胞及胃窦Ｇ细胞数量均有明显的年龄变化；胃肠道Ｇ细胞与ＡＣＴＨＩＲ细胞数量随年龄变化是一致的，而胃肠道与脑垂体ＡＣＴＨＩＲ细胞数量与年龄变化却不相一致；胃肠道的一些杯状细胞可能有分泌ＡＣＴＨＩＲ物质的功能。
OBJECTIVE: To investigate the characteristics of age-related changes of gastrin (G) cells and adrenocorticotropic hormone immunoreactivity (ACTH-TR) cells in the gastrointestinal tract and the pituitary ACTH-IR cell age Change the relationship. Methods: PAP immunohistochemistry and gold immunoelectron microscopy were used. Results: The numbers of ACTHIR cells in gastric antrum and duodenum and pituitary in 18-month rats were significantly lower than those in other groups (P <005; P <001). 2 5 months duodenum ACTH IR and gastric antrum G cells for the highest number, the highest number of pituitary ACTH IR cells for 35 days in rats. In addition, in the gold immunoelectron microscopy, we can see duodenal goblet cells containing gold particles. Conclusions: The numbers of ACTH-IR cells and gastric G-cells in duodenum and pituitary of rats all have obvious age changes. The numbers of G-cells and ACTH-IR cells in gastrointestinal tract are consistent with the changes of age, And the number of pituitary ACTH IR cells and age changes are not consistent; some goblet cells in the gastrointestinal tract may have the function of secretion of ACTH IR material.