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目的研究胎儿血红蛋白(HbF)对单个核细胞的标记情况,并用于孕妇外周血中胎儿有核红细胞(NRBCs)的分选,同时探讨NRBCs的比例与孕周的关系。方法2003-10-2004-04深圳市人民医院选用胎儿特异性抗体HbF对90例正常孕妇外周血、15例未孕妇女和15例胎儿脐血单个核细胞进行标记,并结合流式细胞技术(FACS)对胎儿有核红细胞进行分选。结果孕8~41周的孕妇外周血中HbF阳性标记率为(4·62±3·42)%,与未孕组及胎儿脐血组比较,差异均有极显著性意义(P<0·01)。经流式细胞仪分选出的孕妇外周血中的胎儿NRBCs数在孕12~18周、19~23周最高,分别为412·4±135·5和454·2±153·4。结论早、中、晚期孕妇外周血中均存在胎儿有核红细胞,单抗HbF可以有效地进行识别,利用该细胞进行无创伤性产前诊断的最佳时间宜在妊娠12~23周左右。
Objective To study the labeling of mononuclear cells by fetal hemoglobin (HbF) and to identify fetal nucleated erythrocytes (NRBCs) in peripheral blood of pregnant women and to explore the relationship between NRBCs proportion and gestational age. Methods 2003-10-2004-04 Shenzhen City People’s Hospital selected fetal specific antibody HbF 90 normal pregnant women in peripheral blood, 15 cases of pregnant women and 15 cases of fetal cord blood mononuclear cells were labeled and combined with flow cytometry ( FACS) sorting of fetal nucleated red blood cells. Results The positive rate of HbF in peripheral blood of pregnant women with gestational age from 8 to 41 weeks was (4.62 ± 3.42)%, which was significantly different from that of non-pregnant group and fetal cord blood group (P0.01 01). The number of fetal NRBCs in the peripheral blood of pregnant women sorted by flow cytometry was the highest at 12 to 18 weeks of pregnancy and the highest at 19 to 23 weeks, which were 412.4 ± 135.5 and 454.2 ± 153.4, respectively. Conclusion Fetal nucleated erythrocytes are present in the peripheral blood of pregnant women in the early, middle and late stages. The monoclonal antibody HbF can be effectively identified. The optimal time for noninvasive prenatal diagnosis using this cell should be around 12-23 weeks of gestation.