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目的 对氨水的胃粘膜适应性细胞保护作用进行了研究 ,并对其作用机制进行初步探讨。方法 采用不同浓度氨水在不同时间点对小鼠灌胃给药 ,观察氨水胃粘膜同源细胞保护作用的时———效关系及量———效关系 ;利用无水乙醇造成小鼠急性胃粘膜损伤 ,观察不同浓度氨水对无水乙醇损伤的交叉细胞保护作用 ;提前给与前列腺素合成酶抑制剂———消炎痛、一氧化氮合成酶抑制剂———N -硝基 -L -精氨酸甲酯及感觉神经“切除剂”———大剂量辣椒素 ,观察这些药物对氨水的胃粘膜同源细胞保护作用的影响。结果 15、30mmol·L-1氨水对于胃粘膜具有显著的同源细胞保护作用 ,15、30、6 0mmol·L-1氨水均未呈现出对抗无水乙醇急性胃粘膜损伤的交叉细胞保护作用。N -硝基 -L -精氨酸甲酯、消炎痛及辣椒素可显著对抗氨水的胃粘膜同源细胞保护作用。结论 氨水具有胃粘膜同源细胞保护作用 ,这种作用可能与其激活感觉神经、前列腺素及一氧化氮通路有关
Objective To study the adaptive cytoprotective effect of ammonia on the gastric mucosa and discuss its mechanism of action. Methods The mice were given intragastric administration with different concentrations of ammonia at different time points to observe the time-effect relationship and quantity-effect relationship of the protective effect of ammonia-stomach mucosa homologous cells; Mucosal injury, to observe the cross-cell protective effect of different concentrations of ammonia on absolute ethanol injury; to give prostaglandin synthetase inhibitor—indomethacin, nitric oxide synthase inhibitor—N-nitro-L—in advance. Arginine methyl ester and sensory nerve “resection agent” --- large doses of capsaicin, to observe the effect of these drugs on the protection of gastric mucosa homogenous cells of ammonia. RESULTS: Aqueous ammonia of 15, 30mmol·L-1 had significant homologous cytoprotective effect on gastric mucosa, and 15, 30, and 60 mmol·L-1 ammonia did not show cross-cell protection against acute gastric mucosal injury. N-nitro-L-arginine methyl ester, indomethacin and capsaicin can significantly protect the gastric mucosa from homologous cytoprotection of ammonia. Conclusion Ammonia has protective effects on homologous cells in the gastric mucosa, which may be related to its activation of sensory nerves, prostaglandins, and nitric oxide pathways.