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目的观察皮肤涂抹火箭煤油(RK)对小鼠细胞免疫的抑制作用。方法建立皮肤迟发超敏反应(DTH)模型,观察皮肤火箭煤油的涂抹剂量与其细胞免疫功能抑制作用之间的关系。阴性对照和阳性对照组ICR雌性小鼠背部涂抹丙酮,火箭煤油组涂抹特定剂量及一定次数的火箭煤油(低剂量:0.5ml/kg;中剂量:1ml/kg;高剂量:2ml/kg),每组6只。通过测定耳肿胀度判定火箭煤油的免疫抑制作用。阳性对照组和各实验组采用1%二硝基氟苯(DNFB)致敏。1不同剂量RK染毒1次:分为阴性对照组、阳性对照组、火箭煤油低剂量组、火箭煤油中剂量组、火箭煤油高剂量组;2同一剂量RK不同染毒次数(1次/d,天数不同):分为阴性对照组、阳性对照组、低剂量RK 1次染毒组、低剂量RK 2次染毒组、低剂量RK 3次染毒组、低剂量RK 4次染毒组、低剂量RK 5次染毒组;3不同剂量RK 5次染毒(1次/d,连续5d):分为阴性对照组、阳性对照组、低剂量RK 5次染毒组、中剂量RK 5次染毒组、高剂量RK 5次染毒组。采用体外培养的淋巴细胞增殖活性实验等方法观察皮肤涂抹RK的细胞免疫抑制持续时间,MTT法观察刀豆蛋白A(Con A)诱导的淋巴细胞增殖活性,流式细胞仪检测外周血T淋巴细胞亚群(CD3+、CD4+和CD8+)和脾淋巴细胞细胞周期。结果 RK 1次染毒能降低DNFB致敏引起的ICR小鼠耳肿胀、脾脏系数升高及胸腺指数降低(P<0.05),RK对小鼠的细胞免疫抑制作用随剂量与染毒次数的增加而增加。RK 1次染毒可以降低Con A诱导的脾淋巴细胞增殖且持续至染毒后20d仍有显著差异(P<0.05),染毒后第10天CD4+/CD8+比值显著降低。结论皮肤染毒RK对小鼠细胞免疫具有明显的抑制效应。
Objective To observe the inhibitory effect of skin rocket kerosene (RK) on cellular immunity in mice. Methods The model of delayed skin hypersensitivity (DTH) was established to observe the relationship between skin rocket kerosene dosage and the inhibition of cellular immune function. The ICR female mice in the negative and positive control groups were smeared with acetone on the back and the rocket kerosene group was given a specific dose of rocket kerosene (low dose: 0.5 ml / kg; middle dose: 1 ml / kg; high dose: 2 ml / kg) 6 per group The immunosuppressive effect of rocket kerosene was determined by measuring ear swelling. The positive control group and each experimental group were sensitized with 1% dinitrofluorobenzene (DNFB). 1 different doses of RK exposure 1: divided into negative control group, positive control group, rocket kerosene low dose group, rocket kerosene middle dose group, rocket kerosene high dose group; 2 the same dose of RK different exposure times (1 / d , Different days) were divided into negative control group, positive control group, low dose RK 1 dose group, low dose RK 2 dose group, low dose RK 3 dose group, low dose RK 4 dose group , Low-dose RK 5 times, and 3 different doses of RK 5 times (once daily for 5 days) were divided into negative control group, positive control group, low-dose RK 5 times, middle dose RK 5 exposure groups, high-dose RK 5 exposure groups. The duration of cellular immunosuppression of dermal smear RK was observed by the experiment of proliferation activity of lymphocytes in vitro. The proliferation activity of lymphocytes induced by Con A was observed by MTT assay. The levels of T lymphocytes in peripheral blood were detected by flow cytometry Subpopulations (CD3 +, CD4 + and CD8 +) and spleen lymphocyte cell cycle. Results Exposure of RK for 1 time reduced the ear edema, the spleen coefficient and the thymus index of ICR mice induced by DNFB (P <0.05). The immunosuppressive effect of RK on mice increased with dose and exposure time Increase. RK exposure for 1 time reduced the proliferation of spleen lymphocytes induced by Con A and continued to be significantly different at 20 days (P <0.05). The ratio of CD4 + / CD8 + decreased significantly on the 10th day after exposure to RK. Conclusion RK exposed to skin has obvious inhibitory effect on cellular immunity in mice.