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高尔基氏法对研究神经细胞及其突起有很大意义,但此法本身具有许多缺点而且掌握较难。操作时往往由于技术不熟练而使组织内产生大量铬银沉淀造成假象,或因血管太多而妨碍对细胞及其突起的全貌观察。为了纠正上述缺点,最初曾按Sehrwald 氏法企图以组织块沾胶的方法消除铬银沉淀。此法是将预染后的组织块放入10%明胶(Gelatine)液内,使组织块为胶液所包围,次将组织块放入银液内,此时所产生的铬银沉淀只能在胶层上沈集而不能在组织内部产生。至组织块不再在银液内起沉淀时,以温水将沾有铬银沉淀的明胶层洗去,然后放入净银内以达消除沉淀之目的。
Gorky’s method is of great significance for the study of neural cells and their processes, but this method itself has many disadvantages and is difficult to master. Operation is often due to unskilled technique and make a large number of chromium deposits within the organization caused by the illusion, or because too much blood vessels impede the appearance of cells and their projections. In order to correct these shortcomings, chromic silver precipitation was first eliminated by Sehrwald’s method in an attempt to form a tissue block. This method is the pre-stained tissue pieces into 10% Gelatine (Gelatine) liquid, so that the tissue block is surrounded by glue, the tissue will be placed in the liquid silver, chromium silver precipitation produced at this time only Sink on the gel layer and can not be produced inside the tissue. When the tissue block no longer deposits in the silver liquid, the gelatin layer with the chromium-silver precipitation is washed with warm water and then placed in the net silver to eliminate the sediment.