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目的 认识新疆出血热（ ＸＨＦ） 病毒不同分离株基因区别的本质，从分子水平揭示其基因结构与功能的关系，寻找传播及流行的原因。方法 对１９６５ 年自我国首例病人及１９８４ 年自蜱分离的两株ＸＨＦ 病毒进行了Ｓ 基因片段的克隆测序和分析。结果 两株病毒核苷酸序列同源性为９６ ．７ ％ ，与已经发表的１９６８ 年分离自我国蜱（ＨＹ１３） 和羊（Ｃ６８０３１） 的两株病毒核苷酸序列同源性均较高（９６ ．７ ％ ～９９ ％ ） ；与其它ＣＣＨＦ 病毒相比Ｓ 基因同源性为７７ ．４ ％ ～９２ ．７ ％ 。结论 计算机绘出的系统发生树状图显示我国分离的４ 株病毒形成一单个群体并进一步分为三组，提示流行源自我国。
Objective To understand the nature of the genetic differences between different isolates of Xinjiang hemorrhagic fever virus (XHF) and reveal the relationship between the gene structure and function at the molecular level and to find out the reasons for the spread and epidemic. Methods The S gene fragment was cloned and sequenced from the first patient in China in 1965 and the two XHF viruses isolated from ticks in 1984. Results The nucleotide sequence homology of the two viruses was 96. 7%, which shared high homology (96.7% -99%) with the published nucleotide sequences of two viruses isolated from ticks (HY13) and sheep (C68031) in 1968. Compared with other CCHF viruses The homology to S gene is 77. 4% ~ 92. 7%. Conclusion The computer generated phylogenetic tree shows that 4 isolates in China form a single group and further divided into three groups, suggesting that the epidemic is from China.