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应用细胞培养技术，在离体的正常和牙周病变根片上培养牙周膜细胞（ＰＤＬＣ），经２４ｈ和７２ｈ分别观察其各自的生长状态，并观察纤维粘连蛋白（ＦＮ）对细胞附着和生长的影响，通过细胞计数定量比较其附着和增殖程度的差异。结果显示，在离体的牙周病患牙根面上ＰＤＬＣ附着和增殖数量明显低于正常牙根面，细胞生长状态也较差；经ＦＮ作用后，其附着数量明显增加。进一步证实ＦＮ对ＰＤＬＣ确实是一种有效的促生长因子；此外，还提示ＦＮ的促附着和促增殖作用有所差异，表现在促附着效应更为显著
Periodontal ligament cells (PDLC) were cultured on normal and periodontal diseased roots in vitro by using cell culture technique. The growth of PDLC was observed at 24h and 72h respectively. The adhesion and growth of fibronectin (FN) The influence of cell proliferation was quantified by cell counting. The results showed that the number of PDLC attachment and proliferation on the root surface of periodontal disease patients with PD was significantly lower than that of normal tooth root surface, and the cell growth was also poor. After FN treatment, the number of PDLC attachment and proliferation significantly increased. FN was further confirmed that PDN is indeed a potent pro-growth factor; In addition, FN also prompted the role of promoting adhesion and promoting proliferation were different, the performance of the adhesion effect was more significant