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试验了山金柑 (FortunellahindsiiOsb .cv.swingle)、Murcott桔橙 (CitrusreticulataBlanceC .sinensisOsb.cv.murcott)、伏令夏橙 (C .sinensisosb.cv.valencia)、伏令夏橙和宁波金柑的体细胞杂种 (C .sinensis+F .Crassifoliaswinglecv.Meiwa) 4种柑桔原生质体及其叶片经高温处理后的原生质体活力、电解质渗出率 ( % )的变化 ,配合logsitic方程分别求出各自的高温半致死温度 (LT50 ) ,以此来衡量各品种耐热力的大小。发现原生质体的耐热力大小与叶片耐热力的大小有显著相关性 ,回归方程为Y =1 .3 0 0 7+0 .9881X(r=0 .9771 )。讨论了原生质体耐热力测定的生理基础
Citrus reticulata BlanceC. SinensisOsb.cv.murcott, C. sinensisosb.cv.valencia, solanum nigrum and kumquat were tested for the somatic hybrids of Fortunella hsienii Osb. Cv.swingle, (C. sinensis + F.Crassifoliaswinglecv.Meiwa) protoplasts and leaves of protoplasts after high temperature treatment of protoplasts vitality, electrolyte exudation rate (%) changes, with logsitic equations were obtained for each of the high-temperature semi-lethal Temperature (LT50), in order to measure the size of the variety of heat resistance. The results showed that there was a significant correlation between the heat resistance of protoplasts and the heat tolerance of the leaves. The regression equation was Y = 1.3007 + 0.9881X (r = 0.9761). The physiological basis of the protoplast thermostability assay was discussed