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采用改进的异硫氰酸胍／氯化铯超离心法，从人脑恶性胶质瘤组织中分离总ＲＮＡ，ＯＤ２６０ｎｍ／ＯＤ２８０ｎｍ为２．０２。甲醛变性凝胶电泳、溴化淀染色显示，２８ｓ和１８ｓｒＲＮＡ条带清晰，且２８ｓ条带的荧光强度约为１８ｓ条带的２倍。再经Ｏｌｉｇｏ（ｄＴ）－Ｃｅｌｌｕｌｏｓｅ亲和层析纯化得到ｍＲＮＡ。Ｎｏｒｔｈｅｒｎ转移印迹分析表明，用该方法所分离得到的ｍＲＮＡ无降解，完整性好，核酸纯度完全符合进一步实验研究（如进行反转录合成ｃＤＮＡ等）的要求。
Total RNA was isolated from human glioblastoma tissue using an improved guanidinium isothiocyanate / cesium chloride ultracentrifugation method with an OD260nm / OD280nm of 2.02. Formaldehyde denaturing gel electrophoresis and brominated starch staining showed that the bands of 28s and 18srRNA were clear, and the fluorescence intensity of 28s band was about twice of that of 18s band. The mRNA was purified by Oligo (dT) -Cellulose affinity chromatography. Northern blot analysis showed that the mRNA isolated by this method had no degradation and integrity, and the purity of the nucleic acid was completely in line with the requirements of further experimental studies (such as reverse transcription to synthesize cDNA).