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以分枝虫草(Cordyceps ramosa)菌丝生物量作为指标,研究不同碳源和氮源对分枝虫草液体培养的影响,采用正交设计试验法筛选最佳液体培养基配方,并分析菌丝主要营养成分。结果表明,分枝虫草液体培养基的最佳碳源为甘油,最佳氮源为硝酸钾,最佳配方是:甘油35 g·L-1、硝酸钾12 g·L-1、酵母浸膏12 g·L-1、麦芽浸出物10 g·L-1、H2O 1 L;其菌丝的粗蛋白、粗脂肪和总灰分含量分别为32.90%、1.20%和5.89%,其腺苷、多糖和虫草酸的含量分别为0.805%、5.89%和5.61%;含有17种氨基酸,总含量为23.97%;微量元素铁、锰、铜、锌和硒含量分别为76.70 mg·kg-1、5.10 mg·kg-1、3.70 mg·kg-1、33 mg·kg-1和0.176 mg·kg-1,重金属铅、砷、汞的含量均符合国家食用菌相关标准。分枝虫草的液体培养可作为分枝虫草开发利用的重要途径。
The mycelial biomass of Cordyceps ramosa was used as an index to study the effects of different carbon sources and nitrogen sources on the liquid culture of Cladocin crenulate. The optimum liquid medium was screened by orthogonal design test, nutrient content. The results showed that the optimal carbon source for the liquid culture medium of Cladoniae Cinnamomi was glycerol and the best nitrogen source was potassium nitrate. The optimal formula was: glycerol 35 g · L-1, potassium nitrate 12 g · L-1, yeast extract 12 g · L-1, malt extract 10 g · L-1, H2O 1 L. The mycelia crude protein, crude fat and total ash content were 32.90%, 1.20% and 5.89% The contents of trace elements iron, manganese, copper, zinc and selenium were 76.70 mg · kg -1 and 5.10 mg · kg -1, respectively. The contents of trace elements iron, manganese, copper, zinc and selenium were 0.805%, 5.89% and 5.61% · Kg-1, 3.70 mg · kg-1, 33 mg · kg-1 and 0.176 mg · kg-1. The content of heavy metal lead, arsenic and mercury all met the national edible fungus related standard. Cladding Cordyceps liquid culture can be used as an important way to develop and utilize Cordyceps.