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目的建立测定人血浆中阿比朵尔浓度的HPLC法并研究阿比朵尔的药动学。方法色谱柱为Hypersil C4(150 mm×4.6 mm,5μm),流动相为甲醇-0.05 mol.L-1磷酸二氢铵缓冲液-三乙胺(体积比为60.0∶40.0∶0.4,pH 3.5),检测波长为315 nm,吲哚美辛作为内标,测定人血浆中的阿比朵尔药物浓度。结果阿比朵尔质量浓度线性范围为0.01~2.00 mg.L-1,回归方程为Y=2.169ρ+0.029,r=0.9992,回收率大于85%,日内和日间RSD均小于15%。结论HPLC法适用于阿比朵尔药动学研究以及临床上血药浓度监测。
Objective To establish a HPLC method for the determination of abidol in human plasma and study the pharmacokinetics of abidol. Methods The column was Hypersil C4 (150 mm × 4.6 mm, 5 μm) and the mobile phase was methanol-0.05 mol·L-1 ammonium dihydrogen phosphate buffer-triethylamine (volume ratio was 60.0:40.0:0.4, pH 3.5) , The detection wavelength was 315 nm, and indomethacin was used as an internal standard to determine the drug concentration of abiraterone in human plasma. Results The linear range of Abbott concentration was 0.01-2.00 mg.L-1. The regression equation was Y = 2.169 ρ + 0.029, r = 0.9992. The recovery was more than 85%. The intra-day and inter-day RSD were less than 15%. Conclusion HPLC method is suitable for arbidol pharmacokinetic studies and clinical monitoring of plasma concentrations.