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运用血清药理学方法，取新生小鼠大脑皮层进行体外神经细胞培养，建立离体的神经细胞谷氨酸损伤模型，通过形态学观察、ＭＴＴ 自动微量法研究“李氏５ 号方”对原代培养皮层神经细胞谷氨酸损伤的影响。研究结果表明“李氏５ 号方”水煎剂（ 含挥发油成分） 虽能抑制原代培养皮层神经细胞损伤，但作用不显著，而“李氏５ 号方”含药血清则能不同程度地抑制谷氨酸介导的神经损伤，并呈剂量依赖性。提示“李氏５ 号方”可拮抗兴奋性氨基酸神经毒性
Using serum pharmacology methods, neonatal mouse cerebral cortex was cultured in vitro to establish an in vitro glutamate injury model of nerve cells. Through morphological observation and MTT automatic trace method, “Lee’s No. 5 Formula” was studied for the primary The effect of glutamate injury on cultured cortical neurons. The results showed that the “Lee’s No. 5 Formula” decoction (containing volatile oil components) can inhibit the damage of primary cultured cortical neurons, but the effect is not significant, and the “Li’s No. 5 Formula” containing serum can be to varying degrees. Inhibition of glutamate-mediated nerve injury was dose-dependent. Prompt that “Li’s Formula 5” can antagonize excitatory amino acid neurotoxicity