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目的：克隆人淋巴毒素ｃＤＮＡ并测序。方法：采用ＲＴ－ＰＣＲ技术，从ＰＨＡ／ＰＭＡ活化的人Ｔ细胞系Ｊｕｒｋａｔ细胞总ＲＮＡ中扩增人淋巴毒素ｃＤＮＡ，并定向克隆于ｐＵＣ１８、ｐＵＣ１９质粒载体，Ｓａｎｇｅｒ双脱氧链终止法测序。结果：ＲＴ－ＰＣＲ扩增出一个５４１ｂｐ的ＤＮＡ片段，限制性内切酶图谱分析和测序结果显示，该５４１ｂｐ片段为编码人淋巴毒素成熟肽的ｃＤＮＡ，与公布的人淋巴毒素ｃＤＮＡ序列完全一致。结论：本实验成功地克隆了人淋巴毒素ｃＤＮＡ，为在大肠杆菌表达人淋巴毒素并进一步研究其功能，以及淋巴毒素的开发与临床应用奠定了基础。
Objective: To clone and sequence human lymphotoxin cDNA. METHODS: Human lymphotoxin cDNA was amplified from total RNA of PHA / PMA-activated human T cell line Jurkat by RT-PCR and cloned into pUC18 and pUC19 plasmid vector for sequencing by Sanger dideoxy chain termination method. Results: A 541bp DNA fragment was amplified by RT-PCR. The restriction endonuclease analysis and sequencing showed that the 541bp fragment was cDNA encoding human lymphotoxin mature peptide, which was identical to the reported human lymphotoxin cDNA sequence. Conclusion: This study successfully cloned human lymphotoxin cDNA, which laid the foundation for the expression of human lymphotoxin in E. coli and further study of its function, as well as the development and clinical application of lymphotoxin.