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经病原菌的分离、纯化、人工感染和生理生化测定 ,认为变异微球菌为牙鲆出血性败血病的病原菌。菌体为圆形或椭圆形 ,单个、成对排列或成短链、四联及不规则的堆团。单个菌大小为 0 .5～ 1.8μm。无鞭毛 ,不运动 ,无牙孢 ,无荚膜。革兰氏阳性 ,不抗酸。好氧菌、葡萄糖 O- F试验为氧化型。测定的2 2种糖苷醇类中 ,仅葡萄糖、D-果糖、蔗糖、麦芽糖、甘油为好氧产酸 ,D-甘露糖弱产酸 ,且均不产气。氧化酶、过氧化氢酶阳性 ,硝酸盐还原、美兰还原为阳性。甲基红、V- P反应为阳性。能水解明胶、马尿酸钠 ,不能水解七叶苷、淀粉。不产生硫化氢、吲哚。不能利用柠檬酸盐、丙二酸盐。精氨酸双水解酶、赖氨酸脱羧酶、鸟氨酸脱羧酶、脲酶阴性 ,卵磷脂酶、凝固酶阳性。不溶血。先锋霉素、氯霉素、青霉素、红霉素为该病原菌的敏感药物
Isolation, purification, artificial infection and physiological and biochemical determination of the pathogenic bacteria, that the mutant Micrococcus luria bleeding turbid septicemia pathogens. Cells are round or oval, single, arranged in pairs or into short chain, quadruple and irregular pile. Single bacteria size is 0.5 ~ 1.8μm. No flagella, no exercise, no toothpaste, no capsule. Gram-positive, non-acid. Aerobic bacteria, glucose O-F test is oxidized. Of the 22 glycosidic alcohols tested, only glucose, D-fructose, sucrose, maltose, and glycerol were aerobic acidogenic and D-mannose weakly acidic, and none of them produced any gas. Oxidase, catalase positive, nitrate reduction, Meilan reduction was positive. Methyl red, V-P reaction was positive. Hydrolyzable gelatin, sodium hydrogen urate, can not hydrolyzed aesculin, starch. Does not produce hydrogen sulfide, indole. Can not use citrate, malonate. Arginine hydrolase, lysine decarboxylase, ornithine decarboxylase, urease negative, lecithinase, coagulase positive. Not hemolytic. Vanguard ADM, chloramphenicol, penicillin, erythromycin sensitive drugs for the pathogen