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目的:观察大鼠心肌梗死急性期使用宁心颗粒心肌内乙酰胆碱酯酶(Ach E)的活性。方法:选取清洁级SD大鼠86只,按照既往文献方法制作大鼠心肌梗死模型,术后存活的大鼠随机分为假手术组、模型组、美托洛尔组、中药大剂量组和正常剂量组。分别相应给药30d后,从各组大鼠心脏梗死区周围区域相同位置取材,用实时定量PCR法检测心肌中Ach E mRNA的水平。结果:心肌梗死后,模型组大鼠心肌内Ach E mRNA的水平相比假手术组显著升高(P<0.01),施以药物30d后,与模型组比较,中药组的Ach E mRNA水平显著降低(P<0.01)。而美托洛尔组则无明显差异。中药大剂量、正常剂量组间无明显差异。结论:心肌梗死急性期发生恶性心律失常几率较高。检测宁心颗粒用药前后心肌中乙酰胆碱酯酶的活性变化,可以了解药物对心梗后重构的迷走神经的影响。
Objective: To observe the activity of acetylcholinesterase (Ach E) in Ningxin Granule in the acute stage of myocardial infarction in rats. Methods: Totally 86 SD rats were selected to establish a rat model of myocardial infarction. The rats survived were randomly divided into sham-operation group, model group, metoprolol group, high-dose group and normal Dose group. After correspondingly administrated for 30 days respectively, the AchE mRNA level in myocardium was detected by real-time quantitative PCR at the same position around the infarct area of rats in each group. Results: After myocardial infarction, the level of Ach E mRNA in model group was significantly higher than that in sham operation group (P <0.01). Compared with model group, Ach E mRNA level in model group was significantly increased Decreased (P <0.01). The metoprolol group no significant difference. Chinese medicine high-dose, normal dose group no significant difference. Conclusion: The incidence of malignant arrhythmia in acute myocardial infarction is high. The changes of acetylcholinesterase activity in myocardium before and after the treatment of Ningxin Granule can be used to understand the effect of drugs on vagal nerve remodeling after myocardial infarction.