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目的 探讨膀胱癌新的生物疗法。方法 采用盐析法提取可溶性膀胱癌抗原 ,联合卡介苗在体外诱导正常人外周血单核细胞 ,产生细胞毒T淋巴细胞 (CTL )。MTT法测定其体外细胞毒活性 ,并观察抑制裸鼠体内移植瘤生长的情况。结果 在体外 ,CTL对膀胱癌细胞和卵巢癌细胞的杀伤活性分别是 ( 96.3± 1.4) %和 ( 14 .2± 1.9) % (P <0 .0 1)。在体内 ,CTL组和未经处理的对照组比较 ,膀胱癌细胞移植到裸鼠体内的第 10天 ,肿瘤体积分别为 ( 4 0 .4± 18.2 )mm3 和 ( 2 2 0 .3± 3 1.2 )mm3 (P <0 .0 1) ,裸鼠平均生存期分别为 3 2 .5天和 16.3天 (P <0 .0 1)。结论 膀胱癌抗原和卡介苗诱导产生的CTL ,在体外和体内均有较强的细胞毒作用 ,能明显抑制肿瘤生长。
Objective To investigate new biological therapy of bladder cancer. Methods Soluble bladder cancer antigen was extracted by salting - out, and BCG was induced in vitro to induce the production of cytotoxic T lymphocytes (CTLs). MTT assay cytotoxicity in vitro, and observe the inhibition of tumor growth in nude mice in vivo. Results The killing activity of CTL on bladder cancer cells and ovarian cancer cells in vitro was (96.3 ± 1.4)% and (14.2 ± 1.9)% (P <0.01), respectively. In vivo, CTL group and untreated control group, the bladder cancer cells were transplanted into nude mice on the 10th day, the tumor volume were (40 ± 18.2) mm3 and (20.3 ± 3 1.2 ) mm3 (P <0.01). The average survival time of nude mice was 32.5 days and 16.3 days (P <0.01), respectively. Conclusion CTL induced by bladder cancer antigen and BCG have stronger cytotoxicity both in vitro and in vivo, which can significantly inhibit tumor growth.