论文部分内容阅读
新霉素的效价测定系依据生物法。作者等寻求了其简易的比色分析法:取试品溶液5ml.(相当于~(50-400r/ml.)试品),置玻栓试管中,加2%月桂酸的异戊醇溶液5ml.及5%碳酸钠溶液0.5ml,将此混合液猛为振摇5分钟并离心分离。移取上层异戊醇液4ml.于另一玻栓试管中,加蒸馏水50%醋酸液0.2ml,将此混合液振摇5分钟。移取下层水液4ml.于另一玻栓试管中,加20%碳酸钠溶液1ml.并经振摇,将此混合液在冰水中冷却,加1% 1,2-萘醌-4-磺酸钠溶液0.5ml.于0—2℃的冰水中放置20分钟。于此混合液中加丙酮——乙醇(1:1)的混合液2ml.,振摇2分钟后继加50%
The determination of neomycin potency is based on the biological method. The author sought its simple colorimetric assay: Take 5 ml of the test solution (equivalent to ~ (50-400 r / ml)), place the glass tube in a glass tube, add 2% lauric acid in isoamyl alcohol solution 5ml. And 5% sodium carbonate solution 0.5ml, the mixture was vigorously shaken for 5 minutes and centrifuged. Pipette the upper amyl alcohol solution 4ml in another glass tube bolt, add distilled water 50% acetic acid solution 0.2ml, the mixture was shaken for 5 minutes. Pipette 4ml lower aqueous solution in another glass bolus tube, add 20% sodium carbonate solution 1ml and shake, the mixture was cooled in ice water, add 1% 1,2-naphthoquinone-4-sulfo 0.5ml sodium acid solution in ice water at 0-2 ℃ for 20 minutes. In this mixture was added acetone - ethanol (1: 1) mixture 2ml, shake 2 minutes followed by 50%