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杜氏藻属(Dunaliella)是绿藻门的一类极端耐盐的单细胞藻。本文通过核基因ITS序列和叶绿体基因rbcL序列确定了一株分离自舟山群岛的杜氏藻D3的分类地位,并比较了4种NaCl浓度对该藻的生长、蛋白质含量、总脂含量和叶绿素荧光参数的影响,以期为该藻在今后的科研与应用提供基础资料。结果表明,该藻与23株杜氏藻的ITS序列之间的遗传距离在0.026~0.136之间,与21株同属藻rbcL序列之间的遗传距离在0.005~0.060之间,由遗传距离和聚类图推断该杜氏藻D3属于D.viridis。不同NaCl浓度对该藻D3生长影响表明1.00 mol·L-1 NaCl培养藻生长最快,培养至10 d时,0.44 mol·L-1、2.00mol·L-1和3.00 mol·L-1 NaCl培养藻细胞数分别是它的85.9%、93.2%和80.7%;该藻可溶性蛋白含量在1.00 mol·L-1 NaCl培养最高;藻细胞总脂含量在0.44~2.00 mol·L-1 NaCl浓度范围内随盐度升高总脂含量增加,2.00 mol·L-1 NaCl培养最高(占干重的22.3%);叶绿素荧光参数(Fv/Fm、ФPSII、qp)在高盐(3.00 mol·L-1 NaCl)培养显著降低,而NPQ则升高。
Dunaliella is a class of extremely salt-tolerant single-cell algae from the genus Chlorophyta. In this paper, the taxonomic status of Dunaliella salina D3 isolated from Zhoushan archipelago was determined by ITS sequence and chloroplast rbcL sequence. The effects of NaCl concentration on the growth, protein content, total lipid content and chlorophyll fluorescence parameters In order to provide the basic information for the algae in the future research and application. The results showed that the genetic distance between the algae and 23 Dunaliella ITS sequences ranged from 0.026 to 0.136. The genetic distance between the alga and 23 algae rbcL sequences ranged from 0.005 to 0.060, The figure concluded that Dunaliella D3 belongs to D. viridis. The effects of different NaCl concentrations on the growth of this algae D3 showed that the growth of algae cultured in 1.00 mol·L-1 NaCl was the fastest, and at 10 days, 0.44 mol·L-1,2.00 mol·L-1 and 3.00 mol·L-1 NaCl The number of algal cells in culture was 85.9%, 93.2% and 80.7%, respectively. The content of soluble protein in algae was the highest in 1.00 mol·L-1 NaCl and the total lipid content in algae was in the range of 0.44-2.00 mol·L-1 NaCl The content of total lipids increased with the increase of salinity, and the highest was 2.00 mol·L-1 NaCl (22.3% of the dry weight). The chlorophyll fluorescence parameters (Fv / Fm, ΦPSII, qp) 1 NaCl) significantly decreased while NPQ increased.