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目的探讨痛相关情绪模型大鼠前扣带皮层(ACC)磷酸化细胞外信号调节激酶(p-ERK)的分布特点。方法将12只雄性SD大鼠随机分为对照组和模型组。模型组大鼠进行右侧腰5脊神经结扎制做模型。采用右后足跖机械痛阈检测观察行为变化,旷场实验和高架O迷宫实验检测痛相关情绪变化,免疫荧光技术检测同侧前扣带皮层前囟前3.2、2.7及2.2mm 3个水平p-ERK表达。结果大鼠经神经病理痛模型制做成功后机械痛阈显著下降,焦虑样行为产生。前扣带皮层前囟前3.2、2.7及2.2mm水平p-ERK阳性细胞表达量分别为11.89±2.57、32±4.67和17.56±2.04。对照组相应的p-ERK阳性细胞表达量分别为12.44±2.16、10±0.87和10.11±1.36。除前囟前3.2mm水平对照组与模型组相比没有显著性差异(P>0.05)之外,其他两个水平p-ERK阳性细胞表达量模型组显著高于对照组(P<0.01)。结论神经病理性疼痛能诱发大鼠焦虑情绪的产生及ACC脑区pERK的表达增高,这种变化可能主要与ACC脑区前囟前2.7及2.2mm水平p-ERK的变化相关,而与前囟前3.2mm水平无关。
Objective To investigate the distribution of phosphorylated extracellular signal-regulated kinase (p-ERK) in the anterior cingulate cortex (ACC) of rats with pain-related emotion. Methods Twelve male SD rats were randomly divided into control group and model group. The rats in the model group were subjected to right lumbar 5 spinal nerve ligation to make a model. The right hind paw metatarsal mechanical pain threshold test was used to observe the behavioral changes. The open field test and elevated O-maze test were used to detect the pain-related changes in mood. The levels of 3.2, 2.7 and 2.2 mm before ipsilateral anterior cingulate cortex were detected by immunofluorescence -ERK expression. Results The rat model of neuropathic pain made a significant reduction in mechanical pain threshold and anxiety-like behavior. The expression of p-ERK positive cells at the anterior cingulate cortex of 3.2, 2.7 and 2.2 mm before bregma was 11.89 ± 2.57, 32 ± 4.67 and 17.56 ± 2.04, respectively. The corresponding p-ERK positive cells in the control group were 12.44 ± 2.16, 10 ± 0.87 and 10.11 ± 1.36, respectively. The levels of p-ERK positive cells in the other two groups were significantly higher than those in the control group (P <0.01), except that there was no significant difference between the control group before the bregma and the model group (P> 0.05). CONCLUSIONS: Neuropathic pain can induce the anxiety in rats and increase the expression of pERK in ACC. The changes may be mainly related to the changes of p-ERK at 2.7 and 2.2 mm before anterior bregma in the brain, 3.2mm level has nothing to do.