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燕麦是重要的粮饲兼用作物,构建燕麦EMS突变体库对燕麦功能基因组学研究和遗传改良有重要意义。本试验利用化学诱变剂甲基磺酸乙酯(EMS,ethyl methane sulfonate)处理燕麦品种花早2号,获得了4083株M1材料;对其中2000个单株种植了M2株行,进行全生育期调查,鉴定其表型变化;对2份黄化苗突变材料种植了M3家系,进行相关突变性状的稳定性验证。结果表明,燕麦经EMS处理后代变异巨大,在M2发现表型突变材料196份,变异率为9.8%,变异类型非常丰富,包括幼苗习性、叶片性状、分蘖、株高、穗部形态及成熟期等突变株系。M3证实突变的黄化苗特性可以稳定遗传。本研究建立了燕麦EMS诱变体系,获得的燕麦变异类型丰富,为燕麦功能基因组学研究和燕麦遗传改良奠定了材料基础。
Oats are important grain-crops and crops. It is important to construct oats EMS mutants library for functional genomics research and genetic improvement of oats. In this study, 4,883 M1 materials were obtained from the treatment of the oat variety Hua-Zao 2 with the chemical methane ethylsulfonate (EMS), and M2 lines were planted in 2000 of them. Period of investigation, identification of its phenotypic changes; 2 of the mutant material of yellowing planted M3 family, the stability of the relevant mutant traits verification. The results showed that there was great variation in the offspring generation of EMS by EMS. There were 196 mutants with phenotypic mutation in the M2, the variation rate was 9.8%, and the types of variation were very rich, including seedling traits, leaf traits, tillers, plant height, ear morphology and maturity Mutant lines. M3 confirmed that the mutant EPO could stably inherit. In this study, oat EMS mutagenesis system was established, and the variety of oats obtained was rich, which laid the material foundation for oats functional genomics research and oats genetic improvement.