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目的采用HPLC-ELSD法建立知母的指纹图谱,全面综合反映和控制知母整体质量。方法 Thermo色谱柱C8(250mm×4.6 mm,5μm),流动相为乙腈(A)-0.2%冰醋酸水溶液(B)梯度洗脱:0~5 min,5%~7%A;5~15 min,7%~15%A;15~36 min,15%~22%A;36~43 min,22%~35%A;43~51 min,35%~50%A;51~60 min,100%A;柱温25℃,体积流量1 m L/min,ELSD检测器雾化器(空气)体积流量2.6 L/min,漂移管温度100℃,进样量20μL。结果在60 min内获得了较好分离效果的特征图谱,确定12个主要特征峰作为共有峰,建立了能够使多数化学成分色谱分离的知母药材HPLC-ELSD指纹图谱。结论该方法简便、可靠,并且同时指认了知母中皂苷类和双苯吡酮类成分,能较全面地反映知母整体性的化学成分特征,可用于全面有效地控制知母的质量。
OBJECTIVE To establish the fingerprint of Anemarrhena asphodeloides by HPLC-ELSD and comprehensively reflect and control the whole quality of Anemarrhena asphodeloides. Methods Thermo column C8 (250 mm × 4.6 mm, 5 μm) was used as the mobile phase. The mobile phase consisted of a gradient of acetonitrile (A) -0.2% glacial acetic acid (B): 0-5 min, 5-7% A; , 15% to 15% A, 15% to 36%, 15% to 22% A, 36% to 43%, 22% to 35% A, 43% to 51%, 35% to 50% % A; column temperature 25 ℃, volume flow 1 m L / min, ELSD detector atomizer (air) volume flow 2.6 L / min, drift tube temperature 100 ℃, injection volume 20μL. Results The characteristic chromatogram of better separation effect was obtained within 60 min. Twelve main characteristic peaks were identified as the common peak. HPLC-ELSD fingerprinting of Rhizoma et Radix et Rhizoma Rhei was established to separate most of the chemical constituents. Conclusions The method is simple and reliable, and at the same time, it can also identify the components of saponins and dipyrazid in Anemarrhena asphodeloides, which can comprehensively reflect the chemical constituents characteristics of the whole and can be used to control the quality of Anemarrhena asphodelo as well.