作者曾观察到apo A-I及其脂质体(apoA-Ⅰ:PC)对LDL受体表达有作用,这种作用与其能使细胞内胆固醇外流有关。最近纯化了人血清apoA-Ⅳ,并观察到apoA-Ⅳ脂质体能使细胞胆固醇外流.因此进一步观察该脂质体是否对LDL受体表达也有作用,并与其能使细胞胆固醇外流有关。 ApoA-Ⅳ是从LDS与Intralipid孵育中得到粗品,再经SDS-PAGE、电泳回收及去SDS获得分子量46000的apoA-Ⅳ.脂质体(apoA-Ⅳ:DMPC按)脱氧胆酸透析法制备.家兔成纤维细胞用皮肤活检组 The authors have observed that apo A-I and its liposomes (apoA-I: PC) have an effect on LDL receptor expression in relation to their ability to block intracellular cholesterol efflux. Recently, human serum apoA-IV was purified and it was observed that apoA-IV liposomes can excrete the extracellular cholesterol, so it is further observed whether this liposome has an effect on the expression of LDL receptor and is related to the cholesterol efflux. ApoA-IV was obtained from the incubation of LDS and Intralipid, then apoA-IV with a molecular weight of 46000 was obtained by SDS-PAGE, electrophoresis and desalination. Liposomes (apoA-IV: DMPC) were prepared by deoxycholate dialysis. Rabbit fibroblast skin biopsy group