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目的探讨大鼠消化道是否存在ＧｎＲＨ受体及其定位。方法采用兔抗ＧｎＲＨ抗独特型抗体的免疫组织化学ＡＢＣ法。结果胃底腺壁细胞、胃小凹上皮细胞及肌间神经丛的副交感神经节细胞呈ＧｎＲＨ受体免疫反应性。三段小肠绒毛上皮细胞、潘氏细胞、十二指肠腺上皮细胞以及小肠肌间神经丛副交感神经节细胞，均呈较强的ＧｎＲＨ受体免疫反应性。在盲肠、结肠和直肠，粘膜上皮细胞、肠腺上皮细胞及肌间神经丛呈ＧｎＲＨ受体免疫反应性。阳性物质均分布在胞质内，胞核呈阴性。结论大鼠消化道广泛分布有ＧｎＲＨ受体，其分布模式与先前ＧｎＲＨ及ＧｎＲＨ－ｍＲＮＡ的相类似。说明胃肠道的一些细胞分泌的ＧｎＲＨ可能以自分泌或旁分泌的方式参与消化功能的调节。
Objective To investigate the presence and location of GnRH receptor in the digestive tract of rats. Methods Rabbit anti-GnRH anti-idiotypic antibody immunohistochemical ABC method. Results The parasympathetic ganglion cells in gastric parietal gland cells, gastric pits and myenteric plexus showed GnRH receptor immunoreactivity. Three segments of villus epithelial cells, Paneth cells, duodenal glandular epithelial cells and parasympathetic ganglion cells of small intestine showed strong GnRH receptor immunoreactivity. GnRH receptor immunoreactivity was observed in the cecum, colon and rectum, mucosal epithelial cells, gut epithelial cells and myenteric plexus. Positive substances are distributed in the cytoplasm, the nucleus was negative. Conclusion The GnRH receptor is widely distributed in the digestive tract of rats and its distribution pattern is similar to that of GnRH and GnRH-mRNA. It shows that GnRH secreted by some cells in the gastrointestinal tract may be involved in the regulation of digestive function in an autocrine or paracrine manner.