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目的研究并探讨偶发与复发自然流产孕妇的流产物细胞遗传学异常发生情况,为临床工作提供参考。方法选取2014年3月~2016年12月期间经我院收治的186例早期自然流产孕妇作为本次研究的研究对象,根据其流产发生次数将孕妇分成偶发自然流产组以及复发自然流产组,其中偶发组97例,复发组89例。针对孕妇行清宫手术时采集其流产物绒毛组织,通过原位培养的方法进行绒毛细胞培养并制备染色体、分析染色体核型。结果偶发自然流产组中共培养成功93例,包含异常核型42例(45.16%),异常核型中染色体数目异常比例为92.86%(39/42);复发自然流产组中共培养成功86例,包含异常核型49例(56.98%),异常核型中染色体数目异常比例为91.84%(45/49)。偶发组与复发组孕妇的流产物绒毛细胞染色体核型异常发生率以及异常类型均不存在统计学差异(均P>0.05)。结论孕妇早期流产与其胚胎染色体数目异常存在一定关联,但孕妇流产次数与流产物细胞遗传学异常情况的发生之间不存在显著联系。
Objective To study and discuss the occurrence of cytogenetic abnormalities of abortion products in spontaneous and recurrent spontaneous abortion pregnant women and provide reference for clinical work. Methods 186 pregnant women with early spontaneous abortion treated in our hospital from March 2014 to December 2016 were selected as the study subjects and divided into spontaneous abortion group and recurrent spontaneous abortion group according to the number of their abortions Sporadic group 97 cases, recurrence group 89 cases. The uterus tissue was collected during the operation of the uterus for the pregnant women. The villus cells were cultured by in-situ culture and the chromosomes were prepared. The karyotype of the chromosomes was analyzed. Results 93 cases of spontaneous spontaneous abortion group were successfully cultured, 42 cases (45.16%) had abnormal karyotype and 92.86% (39/42) had abnormal karyotype, 86 cases were successful in recurrent spontaneous abortion group, including Abnormal karyotype in 49 cases (56.98%), anomalous karyotype aberrant chromosome number was 91.84% (45/49). There was no significant difference in the abnormalities of chromosomal karyotypes and the abnormal types of hair loss in pregnant women with sporadic or recurrent group (all P> 0.05). Conclusion There is a correlation between early pregnancy loss and abnormal number of chromosomes in pregnant women. However, there is no significant correlation between the number of miscarriage and the cytogenetic abnormalities of abortion products.