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目的建立简单、高效体外培养C57BL/6小鼠骨髓间充质干细胞(BMSCs)的方法。方法体外分离、培养BMSCs,分为含10%FBS的L-DMEM培养基组(A组)、含15%FBS的L-DMEM培养基组(B组)和含10%FBS的DMEM/F12培养基组(C组)。倒置显微镜下观察细胞形态变化,台盼蓝染色检测细胞活力,MTT法检测细胞生长曲线,流式细胞术鉴定细胞纯度。结果与A组和B组相比,C组BMSCs形态较规则,数量较多,生长状态较好,并且纯度较高。结论含10%FBS的DMEM/F12培养基更适用于C57BL/6小鼠BMSCs的体外培养。
Objective To establish a simple and efficient method for culturing C57BL / 6 mouse bone marrow mesenchymal stem cells (BMSCs) in vitro. Methods BMSCs were isolated and cultured in vitro. The cells were divided into L-DMEM medium containing 10% FBS (group A), L-DMEM medium containing 15% FBS (group B) and DMEM / F12 containing 10% FBS Basal group (C group). Cell morphology was observed under inverted microscope. Cell viability was detected by trypan blue staining. Cell growth curve was detected by MTT assay and cell purity was identified by flow cytometry. Results Compared with group A and group B, the morphology of BMSCs in group C was more regular, larger in number, better in growth state and higher in purity. Conclusion DMEM / F12 medium containing 10% FBS is more suitable for in vitro culture of BMSCs in C57BL / 6 mice.