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目的:确定甘草中角鲨烯的超声波辅助最佳提取条件,并分析甘草粗脂肪的组成。方法:分别研究了料液比、浸泡时间、超声温度、超声时间对角鲨烯得率的影响,采用L9(34)正交试验,对提取工艺进行优选;并采用气相色谱-质谱法(GC-MS)分析了甘草粗脂肪的组成。结果:甘草角鲨烯的最佳提取工艺为料液比1∶15,浸泡时间10 h,超声时间50 min,超声温度50℃;甘草粗脂肪中共分离鉴定出26种成分,角鲨烯含量0.8%。结论:超声法操作简便,开发和利用甘草粗脂肪中的角鲨烯具有较好的社会效益和经济效益。
OBJECTIVE: To determine the best ultrasound-assisted extraction conditions of squalene in licorice and to analyze the composition of crude fat of licorice. Methods: The effects of material to liquid ratio, soaking time, ultrasonic temperature and ultrasonic time on the yield of squalene were studied. The optimum extraction conditions were determined by L9 (34) orthogonal test. Gas chromatography - mass spectrometry -MS) analyzed the composition of licorice fat. Results: The best extraction technology of licorice squalene was solid-liquid ratio 1:15, soaking time 10 h, ultrasonic time 50 min and ultrasonic temperature 50 ℃. 26 components were isolated and identified from licorice crude fat, and the content of squalene was 0.8 %. Conclusion: The ultrasonic method is simple and convenient to develop and utilize squalene in licorice crude fat, which has good social and economic benefits.