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目的探讨β-细辛醚对慢性不可预见性轻度刺激小鼠模型的抗抑郁作用及其机制。方法小鼠随机分为正常组,模型组,β-细辛醚低、中、高剂量组(7.5、15、30mg/kg),氟西汀组(8 mg/kg),边造模边给药3周,通过糖水偏爱实验、强迫游泳实验、旷场实验进行行为学观察,用高效液相荧光检测法检测小鼠中脑和纹状体单胺类神经递质及其代谢产物(5-HT、5-HIAA、DA、DOPAC、HVA、NE)的含量。结果与模型组比较,β-细辛醚中、高剂量组和氟西汀组的糖水偏爱百分比明显增加(P<0.05或P<0.01)、游泳不动时间明显减少(P<0.05),且β-细辛醚中、高剂量组和氟西汀组显著增加中脑、纹状体中5-HT、5-HIAA、DA的含量(P<0.05或P<0.01)。结论通过3周的慢性不可预见性轻度刺激成功建立了小鼠抑郁模型,β-细辛醚具有一定的抗抑郁作用,其机制可能与β-细辛醚能增加中脑和纹状体中5-HT、5-HIAA、DA的含量有关。
Objective To investigate the antidepressant effects and mechanisms of β-asarone on a mouse model of chronic unpredictable mild irritation. Methods Mice were randomly divided into normal group, model group, β-asarone low, medium and high dose groups (7.5,15,30mg / kg), fluoxetine group (8 mg / kg) For 3 weeks, behavioral observation was carried out through the sugar-water preference test, forced swimming test and open-field test. The levels of monoamine neurotransmitters and their metabolites in the midbrain and striatum were detected by high performance liquid chromatography (FCM) HT, 5-HIAA, DA, DOPAC, HVA, NE). Results Compared with the model group, the percentage of glyco-water preference in β-asarone medium and high-dose fluoxetine group was significantly increased (P <0.05 or P <0.01), swimming immobility time was significantly reduced (P <0.05) The contents of 5-HT, 5-HIAA and DA in midbrain and striatum were significantly increased in β-asarone medium, high-dose group and fluoxetine group (P <0.05 or P <0.01). Conclusions The model of depression in mice was established successfully by 3 weeks of chronic unpredictable mild stimulation. Β-asarone has some antidepressant effects. The mechanism may be related to the fact that β-asarone can increase midbrain and striatum 5-HT, 5-HIAA, DA content.