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采用不同的方法接种和利用扫描、透射电镜观察,研究不同致病力的青枯菌对番茄抗病及感病品种根部的吸附、侵入与繁殖。发现番茄抗病品种与感病品种的植株体内在菌体数量上有明显差异,而与青枯菌对番茄根部的吸附关系不显著。电镜观察发现青枯菌强致病力菌株菌体能以游离的形式存在于番茄感病品种根部的细胞间隙中,并能降解植株细胞壁、破坏原生质膜;青枯菌强致病力菌株菌体在抗病品种根内和青枯菌强致病力菌株在抗病及感病品种根内均被番茄植株细胞壁吸附,并且被细胞壁周围的浓密物质所包围。
Different methods of inoculation and utilization of scanning and transmission electron microscopy were used to study the effects of different pathogenic strains of R. solanacearum on the root absorption, invasion and reproduction of tomato disease resistance and susceptible cultivars. It was found that there were significant differences in the number of mycorrhizal fungi in tomato resistant and susceptible cultivars, but not in the roots of tomato with R. solanacearum. Electron microscopy showed that R. solanacearum virulent strains could exist in the form of free form in the intercellular space of the roots of tomato susceptible cultivars and could degrade the cell wall of the plants and destroy the plasma membrane. The strong virulence strains of R. solanacearum In the roots of resistant cultivars and the strong virulence strains of R. solanacearum were all adsorbed by the tomato plant cell wall in the roots of disease-resistant and susceptible cultivars and surrounded by the dense material around the cell wall.