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目的:探讨雷公藤多甙对核因子的影响并为药物治疗银屑病提供理论依据。方法:密度梯度离心法获得外周血单一核细胞(PBMC),体外培养角质形成细胞(KC)。PBMC与KC混合培养,流式细胞仪检测核转录因子κB(NF-κB)表达,ELISA法测上清液白介素-8(IL-8)、细胞间黏附分子-1(ICAM-1)含量。结果:银屑病组NF-κB表达及ICAM-1、IL-8含量与正常对照组间差异有显著性(P<0.01);雷公藤可下调NF-κB表达及IL-8、ICAM-1含量(P<0.01)。结论:雷公藤可能通过影响NF-κB的表达而发挥对银屑病的治疗作用,抑制NF-κB的药物可能有助于银屑病的治疗。
Objective: To investigate the effect of Tripterygium glycosides on nuclear factor and to provide a theoretical basis for drug treatment of psoriasis. Methods: Peripheral blood mononuclear cells (PBMCs) were obtained by density gradient centrifugation and keratinocytes (KCs) were cultured in vitro. PBMC and KC were mixed and cultured. The expression of nuclear factor-kappa B (NF-κB) was detected by flow cytometry. The contents of IL-8 and ICAM-1 were determined by ELISA. Results: The expressions of NF-κB, ICAM-1 and IL-8 in psoriasis group were significantly different from those in normal control group (P <0.01). Tripterygium wilfordii could down-regulate the expression of NF-κB and IL-8 and ICAM-1 Content (P <0.01). Conclusion: Tripterygium wilfordii may play a therapeutic role in the treatment of psoriasis by affecting the expression of NF-κB. The drugs that inhibit NF-κB may contribute to the treatment of psoriasis.