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目的测定补肺活血胶囊中有效成分补骨脂素和异补骨脂素的含量。方法采用超声提取有效成分,色谱柱为Agilent TC-C18,流动相为甲醇-水(48∶52),流速为1.0 m L·min-1,检测波长为320 nm。结果补骨脂素5.9~236μg·m L-1(r=0.9999)和异补骨脂素5.3~212μg·m L-1(r=0.9999)与峰面积的线性关系均良好,平均加样回收率分别为100.37%、97.81%,RSD分别为1.04%、0.84%(n=6)。结论所用方法简便、准确,可作为补肺活血胶囊的质量控制方法。
Objective To determine the content of psoralen and isopsoralen in the active ingredients of Bufei Huoxue Capsule. Methods The active components were extracted by ultrasonic. The column was Agilent TC-C18. The mobile phase was methanol-water (48:52). The flow rate was 1.0 m L · min-1 and the detection wavelength was 320 nm. Results The linear relationship between psoralen 5.9 ~ 236μg · m L-1 (r = 0.9999) and isopsoralen 5.3 ~ 212μg · m L-1 (r = 0.9999) Rates were 100.37% and 97.81%, respectively, with RSDs of 1.04% and 0.84%, respectively (n = 6). Conclusion The method is simple and accurate, which can be used as quality control method of Bufei Huoxue Capsule.